Add scale information (scale bar, image length; in figure/figure legend)
Explain all annotations (in figure/figure legend)
Annotations should be legible (line width, size/point size, color)
Annotations should not obscure key data
Recommended
Annotate imaging details important for interpreting the figure.
(Depending on the main message and imaging technique this may be e.g. image pixel size, imaging intervals (time-lapse in movies), exposure time, or anatomical section.)