Image annotation#
Minimal
Add scale information (scale bar, image length; in figure/figure legend)
Include scale information by incorporating a scale bar within (or outside if insufficient contrast/visibility or obstructs key information, see other checks!) the image to provide a reference for size and distance. Additionally, specify the actual dimensions that the scale bar represents in the figure legend or accompanying text to ensure accurate interpretation of the image’s scale.
Explain all annotations (in figure/figure legend)
Explain all annotations by clearly defining each label, symbol, and color used directly within the figure or in the figure legend. Ensure the figure legend provides comprehensive descriptions to help readers understand the significance and context of each annotation in the image.
Annotations should be legible (line width, size/point size, color)
Annotations should be legible by using an appropriate line width, font size, and point size that ensures clarity and readability. Additionally, choose colors that provide strong contrast against the background and other elements in the image to enhance visibility.
Tutorials
Annotations should not obscure key data
Annotations should be placed thoughtfully to avoid covering or obscuring key data and important features within the image. Ensure that all annotations are positioned in a way that maintains the integrity and clarity of the underlying data, allowing for accurate interpretation.
Recommended
Annotate imaging details important for interpreting the figure
Annotate imaging details critical for interpreting the result directly in the image figure (i.e. scale, labeled biological structure). Depending on the imaging modality this might include also other information such as time stamp for showing frames from time series. Additionally, include information about image processing particulary any non-linear post-processing techniques in the figure legends (e.g. median filter, deconvolution, image reconstruction, inverted LUT).
Other critical information allowing to reproduce the image such as the type of microscope used, objective, magnification, image acquisition settings, and staining methods should be included in the manscript methods.